XRD1

Welcome to XRD1 @ Elettra.

X-rays are an invaluable probe of the structure of matter, and the range of problems where X-rays have proved to be decisive in unravelling a material structure is very wide. The X-Ray Diffraction 1 (XRD1) beamline has been designed (in collaboration with the Istituto di Cristallografia - CNR) primarily for macromolecular crystallography, but the characteristics of the beamline permit to perform a wide variety of experiments. The light source is a multipole wiggler with a useful range from 4 to 21 keV. The optics consist in a vertical collimating mirror, a double-crystal Si(111) monochromator followed by a bendable focussing mirror. The multipole wiggler spectrum includes high photon flux at low energies, allowing the optimization of the anomalous signal of several heavy atoms (up to the calcium edge), and offering the enhancement of the Sulphur anomalous signal. The experimental setup consists in a Huber goniometer with k geometry fully controllable from remote. The beamline hosts small molecules, protein crystallography, powder diffraction, high pressure physics and solid-state experiments.

Research Highlights

Customizing Properties of beta-Chitin in Squid Pen by Chemical Treatments

Loligo vulgaris squid pens (gladii) illuminated using polarized light, showing dichroic effect, a synchrotron X-ray fiber diffraction diagram and an AFM image. The structural organization of β-chitin in the gladius has been studied at different length scales after deproteinization and a partial deacetylation.
Ianiro A et al., Marine Drugs. 2014; 12(12)

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XRD1 Sample Changer

A new Sample Changer (SC) is available on the XRD1 as a result of an in-house development project at ELETTRA. The SC has been designed to handle only SPINE standard sample holders and vials stored into cryo-vial baskets: 5 baskets (i.e. up to 50 samples) can be loaded into the dispensing dewar of the SC where they lie in a liquid nitrogen bath.

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Structure and Morphology of PDI8CN2 for n-Type Thin-Film Transistors

Schematic drawing of the crystal structure of PDI8CN2. 2D-GIXD experimental images of 100nm-thick PDI8CN2 film grown at 120°C and simulated 2D-GIXD image reproduced by using the bulk structure and [001] texturing. Copyright 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Liscio F. et al., Adv. Funct. Mater. 22, 943 (2012)

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Structure of Human NAPE-PLD: Regulation of Bioactive Lipids Biosynthesis by Bile Acids

The secrets of anti-ageing. Longevity and healthy ageing are affected by our diet and lifestyle. EU-funded researchers have associated certain organic compounds called fatty acid ethanolamides (FAEs) with obesity and ageing.
Magotti P et al., Structure 23, 3 (2015)

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Multitarget drug design strategy: tacrine-quinone hybrids designed to block amyloid-ß aggregation and to exert anticholinesterase and antioxidant effects

We have designed, synthesized, and biologically evaluated a series of novel tacrine−naphthoquinone hybrids as anti-AD lead compounds inspired by a multitarget approach aimed to tackle AD on multiple fronts. Nepovimova E et al., J. Med. Chem., Vol. 57 - 20, pp. 8576-8589 (2014)

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Pore flexibility underlies the poor selectivity of CNG channels: a structural, functional and computational analysis.

In contrast with what observed in the rigid potassium channel structures, we saw a significant variation of the pore and the surrounding regions in the presence of different ions, showing that CNG channels are flexible and can accommodate small and large ions with different geometries. Napolitano LMR et al., Proceedings of the National Academy of Sciences, Vol. 112 - 27, pp. E3619-E3628 (2015)

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Expression-Enhanced Fluorescent Proteins Based on Enhanced Green Fluorescent Protein for Super-resolution Microscopy

We present the development of robustly photoswitchable variants of enhanced green fluorescent protein (EGFP), named rsGreens, that display up to 30-fold higher fluorescence in E. coli colonies grown at 37 °C and more than 4-fold higher fluorescence when expressed in HEK293T cells compared to their ancestor protein rsEGFP.
Duwé S et al., ACS Nano, 2015, 9 (10), pp 9528–9541

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Single-Crystal Structure Determination of Hydrogen-bonded Organic Pigments


Chains of hydrogen-bonded molecules (lying on crystallographic [-110] family of planes), π-π stack on top of each other to form a brickwall crystal structure , explaining the favorable charge transport properties. Glowacki ED et al., Advanced Functional Materials, Vol. 25 - 5, pp. 776-787 (2015)

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Inhomogeneity of charge-density-wave order and quenched disorder in a high-Tc superconductor

The hk diffraction pattern shows that resolution-limited streaks connect the Bragg peaks, owing to the formation of Oi stripes in the HgOy spacer layers.

G.Campi et al., Nature 525, 359–362 (2015)

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Cisplatin Encapsulation within the Ferritin Nanocage: A High-Resolution Crystallographic Study

CDDP-encapsulated AFt appears to be an ideal nanocarrier for CDDP delivery to target sites, as it possesses a high biocompatibility and can be internalized by receptor mediated endocytosis thus carrying the drug to tumor tissue with higher selectivity than free CDDP.

N Pontillo et al., Chem. Commun., 2016

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User Area  

Proposal Submission - Macromolecular Crystallography

A new allocation mechanism – dedicated to macromolecular crystallography experiments – has been introduced for XRD1 starting from May 1st 2011.
The following improvements have been implemented:
- no deadline: it is possible to submit a proposal at any time;
- proposal evaluation on a monthly basis;
- reduced dead-time between proposal submission and allocation;
- automatic re-submission in case of overbooking.
We strongly encourage MX users to take advantage of this new procedure.  It will be still possible to apply on a six-month basis only if the experiment requires a particular setup in order to be performed or  extended time is required for bureaucratic purposes such as visa applications.
The beamline is included in the BioStruct-X funding programme for transnational beam-time acces within EU. A parallel funding programme is available for national users too.

Proposal Submission - Other

For experiments different than macromolecular crystallography (no perishable samples and/or particular setup required), the usual six-month based procedure should be followed (look for the User Area in the quick links). The next deadline for proposal submission for beamtime allocation from January 1st to June 30th, 2017 will be September 15th 2016 at 4:30 pm (MET).

Last Updated on Tuesday, 12 April 2016 17:35