Seminars Archive

Microscale Thermophoresis

Abstract
The Technology: the term MicroScale Thermophoresis refers to the directed movement of molecules in microscopic temperature gradients. This thermophoretic movement is determined by the entropy of the hydration shell around the molecules. Almost all interactions between molecules and biochemical process resulting in a change in size, stability and conformation of molecules alters this hydration shell and can be quantified. Such changes allow quantification of binding affinities of proteins, nucleic acids and small molecules as well in standard buffers as in bioliquids (serum, cell lysate).