Insight into the thermal stability of DNA in ionic liquids/water solutions

The utility of ionic liquids (ILs) as alternative solvents for stabilizing and preserving the native structure of DNA over the long term may be envisaged for biotechnological and biomedical applications in the near future.

The delicate balance between the stabilizing and destabilizing effects of IL-mediated interactions
with the structure of DNA is complex and is still not well understood. This work reports a fundamental study dealing with the effect exerted by cations and anions in imidazolium-based ILs on the thermal structural stability of large nucleic acid molecules. Multi-wavelength UV resonance Raman spectroscopy is used for selectively detecting heat-induced structural transitions of DNA localized on specific base tracts.
The results from the present synchrotron-based UVRR investigation contribute to a more comprehensive view of the IL-mediated
interactions with specific tracts of the DNA sequence during thermal unfolding, thus facilitating the design of effective stabilizing ILs for their exploitation in the fields of biomedicine and life science.
 

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Phys. Chem. Chem. Phys., Vol. 23, pp. 15980-15988 (2021) 
Ultima modifica il Venerdì, 06 Agosto 2021 15:40