A multiple-screening platform for investigating single-cell biochemical perturbations upon prion infection
The present work provided novel insight into cellular response upon prion infection evidenced by SR-IRMS, based on the synergy with conventional biological assays as well as fluorescence and atomic force microscopy.
A. Didonna et al., ACS Chem. Neurosci. 2, 160 (2011)
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SR-IRMS was employed to investigate the biochemical perturbations undergone by prion infected mouse hypothalamic GT1-1 cells at the cellular and subcellular level. A decrement in total cellular protein content upon prion infection was identified by whole-cell IR microspectra and validated by bicinchoninic acid assay as well as single-cell volume analysis by atomic force microscopy (AFM). Hierarchical cluster analysis (HCA) of IR data discriminated between infected (Sc-GT1) and uninfected (GT1) cells and allowed to deduce an |
increment of lysosomal bodies within the cytoplasm of infected GT1-1 cells, a hypothesis further confirmed by SR-IRMS at subcellular spatial resolution and fluorescent microscopy. Retrieve articleInfrared Microspectroscopy: A Multiple-Screening Platform for Investigating Single-Cell Biochemical Perturbations upon Prion Infection. A. Didonna, L. Vaccari, A. Bek, G. Legname ACS Chem. Neurosci. 2, 160 (2011) |
