Seminars Archive
Julie L. Hodgkinson
Abstract
Thursday, April 5, 2000, 16:00
Seminar Room, ground floor, Building "T"
Sincrotrone Trieste, Basovizza
Mapping the location and orientation of actin-binding proteins on actin
filaments
Julie. L. Hodgkinson
(Imperial College Sch. Med., National Heart and Lung Institute, London)
ABSTRACT
Often it is not possible to visualise directly the shape and location
of a whole molecule bound to an actin filament in 3D helical image
reconstructions. It is necessary to map the location and orientation
of such molecules using data derived from a combination of
methodologies: for example combining high resolution TEM, 3D helical
image reconstruction,crystallographic and biochemical approaches. Such
an approach will be discussed with reference to calponin.
